Post-biotinylation of Photocrosslinking by Staudinger-Bertozzi Ligation of Preinstalled Alkylazide Tag
نویسندگان
چکیده
منابع مشابه
Post-biotinylation of photocrosslinking by Staudinger-Bertozzi ligation of preinstalled alkylazide tag.
Post-biotinylation of the alkyl azide derivative of trifluoromethyl phenyldiazirine (TPD) was elucidated to apply a photoaffinity biotinylation technique. A photo-modified polyvinilidene difluoride (PVDF) membrane was used as a photolabeled component and we introduced biotin by Staudinger-Bertozzi ligation. The 15 pmol amount of biotinylated reagent was still effective for the visualization of ...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 2005
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.53.1510